About Growing Mushrooms the Easy Way, Volume II
Second Edition, revised and expanded!

Growing Mushrooms the Easy Way, by R.R. Wayne, Ph.D.

What's new in Volume II?

The first volume of the peroxide manual introduced a whole new approach to home mushroom cultivation, one that has gained a reputation for its ease and simplicity. The manual showed how mushrooms could be grown readily without sterile facilities, air filtration, or autoclaves. Now, for growers interested in commercial cultivation, and hobby growers too...

Prepare any quantity of bulk substrate at room temperature.
The second volume of the peroxide manual adds two ground-breaking peroxide methods that can be used to prepare as much substrate as you need without the trouble of heating and cooling.

Just soak your substrate, drain, and inoculate.
The first method is a simple soak-and-drain procedure designed for use with materials such as straw, bagasse, dried grasses, corn stalks, cottonseed hulls, etc. You just load the substrate into a soak chamber, fill it with the appropriate solution at room temperature, soak until the proper moisture content is reached, then drain and inoculate. There's no caustic waste to worry about, no hassle with heating water or steam, and no concern about peroxide-decomposing enzymes in the substrate materials.

Add water and stir.
The other method is an add-and-stir procedure designed for use with peroxide-compatible porous substrates like wood pellet fuel, paper fiber pellets, or (for oyster mushrooms) kiln-dried sawdust . You simply mix all your ingredients together at room temperature, then come back in a couple of hours and inoculate. There's no hurry, though--peroxide keeps the substrate from spoiling. (Refer to Volume I for important information on choosing peroxide-compatible substrates and supplements).

Here's what else you'll find in Volume II:

  • collecting and germinating mushroom spores
  • an alternative to agar medium for mycelial culture and storage
  • screw-cap tubes (slants) as an alternative to Petri dish culture
  • a quick beer-based agar medium
  • re-using disposable Petri dishes
  • preparing "Ten Minute" sawdust spawn in plastic bags
  • preparing "Ten Minute" grain spawn with "instant rice."
  • inoculating straw without spawn
  • sending mycelial cultures in the mail
  • preparing raw wood chips with peroxide
  • Continue on down the page to read the Table of Contents for Volume II. This volume has 23 pages.

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  • Volume II of Growing Mushrooms the Easy Way

    by R. R Wayne, Ph.D.

    Table of Contents


    Acquiring, Storing, and Maintaining Mushroom Cultures

    Using slants instead of agar plates

    The drawbacks of Petri dish culture
    Advantages and disadvantages of slants
    Making peroxide slants
    Making transfers to and from slants
    Cleaning the mycelium with slants

    Beer-Based Medium (BBM)

    Starting with Spores

    Advantages and disadvantages of spore culture
    Peroxide and spores revisited
    Collecting spores
    Germinating spores by the disk method

    Ideas toward mycelial culture without agar

    Why find a substitute for agar?
    How to prepare the plates
    Making transfers
    Cleaning the mycelium
    Storage cultures without agar
    Sending cultures in the mail

    Resterilizing disposable Petri dishes with peroxide

    Spawn Preparation

    Spawn in plastic bags -- "Eight Minute Spawn"

    Advantages and disadvantages of plastic bags
    Making the spawn
    Using the spawn

    Ten Minute grain spawn

    Inoculating straw without spawn

    How to prepare the inoculum
    Notes on the protocol
    The issue of senesence

    Bulk substrate

    Preparing straw with peroxide at room temperature

    Advantages of peroxide preparation of straw
    What about the enzymes?
    Protocol for preparing straw or other drainable substrates
    Notes on the protocol

    Preparing raw wood chips with peroxide

    An "add-an-stir" method of preparing peroxide-compatible substrates

    Advantages of the "Add-and-Stir" method
    The protocol applied to wood pellet fuel at room temperature
    Notes on the protocol
    Natural nitrogen supplements for use with the protocol
    How much water to add
    How much peroxide to use
    Making spawn by by the "add-and-stir" method


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  • This document Copyright 2001 by Randall R. Wayne, Ph.D. All rights reserved. No part of this work may be reproduced or used in any form or by any means without permission of the author.